At Cisbio we provide microplates that help you discover better. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detectionThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR ORE INFORMATION Europe and other ountries 33(0)466 96 0 U. Remove the plate sealer and read on an HTRF® compatible reader . HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. Lyse cells Read on an HTRF compatible reader . Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for. The CLARIOstar® Plus is our most flexible multi-mode plate reader. Lysates are then transferred to the assay plate for the detection of phospho-c-Src (Tyr419) by HTRF. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Interaction with the industry and research communities is essential for innovating! For more than 30 years, Cisbio has been serving the needs of the clinical and drug discovery communities, delivering a world-class portfolio of assays designed to help find answers faster. Make sure the setup for Eu 3+ Cryptate is used. (665 nm and 620 nm) on a compatible HTRF ® reader. Configure your microplate reader. This protocol, HTS designed, allows miniaturization while. → Incubate 2h (if the detection is not sensitive enough, it is better to incubate 20h). 1. Assay plate setup. It can also be further streamlined to TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate. Nephelometers are dedicated instruments that can only detect scattering and are only available as standalone plate readers. 0. Application Area: absorbance, fluorescence, luminescence, and homogeneous time resolved fluorescence assay development. Lysis buffer 1X for two-plate assay protocol on adherent cells Determine the amount of lysis buffer needed for the experiment. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. Incubate overnight at RT. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. FOR HTRF CERTIFIED READER. For more information. FOR HTRF CERTIFIED READER. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The emission of light by the acceptor will be proportional to the level of interaction. HTRF® Europium cryptate donor / Red acceptor readout Setup recommendations for Tristar 5FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER . Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. • Low volume (20µl) • Compatible with all HTRF readers • For cell-based or biochemical assays Trying is believing. FOR HTRF CERTIFIED READER. Figure 1: Principle of HTRF Cortisol competitive assay. Lysates are then transferred to the assay plate for the detection of Phospho-VASP (Ser239) by HTRF. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. * For HTRF microplate recommendations, please visit . • HTRF®-Certified Reader. Preconfigured HTRF. + 4 µL HTRF pre-mixed antibodies. The new ligands are divided into four groups according to their chemical structures and/or interactions with YTHDC1 (Table 1 and Fig. For more information about HTRF. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF Ratio Anti-Tag Antibody Cryptate Inhibitor-Red Inhibitor Purified Tagged Kinase Figure 2: Principle of HTRF kinase competition binding assay (IC 50 - KI determination) HTRF Dasatinib-Red Part # 62KB02REDC & 62KB02REDE Amount: 1 nmol (62KB02REDC) & 20 nmol (62KB02REDE) Concentration: 25 µM in DMSO Form:. Rating: 5. Homogeneous Time-Resolved Fluorescence (HTRF) . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. Our assay development platform relies on PHERAstar readers for absorbance, fluorescence, luminescence, and homogeneous time resolved. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more information about HTRF. HTRF plate reader. Lysates are then transferred to the assay plate for the detection of Total H2AX by HTRF ® reagents. FOR HTRF CERTIFIED READER. For more information about set-up and compatible HTRF ® readers, please visit our website at: ® pre-mixed antibodies TWO- PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells . In particular, these parameters should be entered as below. The ratio of the two fluorescence Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. 3 and higher include optimized reader settings, as well as data analysis, for faster results. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications and can be further upgraded to a multi-mode reader. + 4 µL HTRF ® pre-mixed antibodies . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. culture plate. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. Make sure the setup for Tb Cryptate is used. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Dual-Luciferase® (DLR™) gene assays. Excitation lasers significantly improve performance and lower. Incubate 2 h at RT. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. -CERTIFIED READER IS NEEDED. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. The HTRF ratio obtained represents the total binding. • HTRF®-Certified Reader. TO PREPARE TISSUE SAMPLES: Please refer to the Technical Note “Optimize Your HTRF® Cell Signaling Assays on Tissues” onHow to use Synergy microplate readers for HTRF detection, a versatile technology for drug discovery and life science research. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. PerkinElmer EnVision Multimode Plate Reader. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The sample is then added and incubated 1. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER For more information about HTRF. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. n HTRF plate validated (QC with reference HTRF assays) n Low to high volumes (10 µL to 350 µL) n Can be used with all HTRF compatible readers n For cell-based or biochemical assays Trying is Believing! Plate Characteristics HTRF® 96 - well low volume plate CulturPlateTM 96 - well Microplate CulturPlate TM 384 - well Microplate ProxiPlateTM 384 - Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Evaluation of the performance of the Spark™ multimode reader for far red fluorescence emission. It can also be further streamlined to a one-. Provide more sensitive and precise results. * For HTRF microplate recommendations, please visit . STANDARD . Revvity. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-2 (Ser473) by HTRF. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. + 4 µL HTRF pre-mixed antibodies. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. 0. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. For more information about HTRF. e. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. Seal the plate and incubate 1 hour at RT : Remove the plate sealer and read on an HTRF® compatible reader . 5 hours before a further 3 washing steps. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYDetection. FOR HTRF CERTIFIED READER. Plate cells Stimulate cells 3. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. The optical systems use two scanning All the benefits of HTRF with ELISA plate ease of use. Endpoint mode experiments measure each well of the microplate only once. 1. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Detection plates from providers not listed on this. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The PHERAstar ® FSX high-throughput screening microplate reader uses unique, easy-to-configure optic modules. This protocol, HTS designed, allows miniaturization while. Small ONE- PLATE ASSAY PROTOCOL: overnight FOR HTRF CERTIFIED READER . 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. If not, our scientific team will help you choose the instrument that best suits your needs and budget. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. Push the screw down and turn it clockwise to lock the plate. PURCHASE SEPARATELY . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Lysates are then transferred to the assay plate for the detection of total-p53 by HTRF. e. * For HTRF microplate recommendations, please visit binding of the fluorescent ligand to the receptor, TR-FRET occurs and is measured with an HTRF-certified microplate reader. It features a modular design that allows users to add or change detection cartridges according to their needs. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. For more information about microplate recommendations, please visit our website STORAGE AND STABILITY• HTRF®-Certified Reader. For more information about HTRF. Highly compatible plates for HTRF. BMG LABTECH microplate reader; Early passage Human Osteoblast cells (HOb 406-05a), supplied by the European Collection of Cell Cultures ECACC were seeded onto substrates A and B at a density of 10 4 per sample. 5 EXAMPLE OF MAP PLATE . Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. SV detection plate. The plate was covered and incubated for one hour at room temperature. Finding a plate reader that offers compatibility with microplates sold by multiple leading vendors can protect both the long-term cost and the performance of assays. FOR HTRF CERTIFIED READER. CLARIOstar is equipped with a specific optical device which enables the measurement of. The kit includes reagents, buffers and a microplate. For more information about HTRF ®Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. FOR HTRF CERTIFIED READER For more. FOR HTRF CERTIFIED READER. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. Thanks to HTRF signal stability, the assay plate can be used to check several instruments, or different reader. Assay plates were incubated at RT and read at different time points thereafter on an HTRF ® certified. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. For more. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. • HTRF®-Certified Reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). Make sure the setup for Eu3+ Cryptate is used. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. VICTOR® Nivo™ multimode plate reader 2 | For research use only. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. FOR HTRF CERTIFIED READER. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. FOR HTRF CERTIFIED READER. Customer Transformation Hub. (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). FOR HTRF CERTIFIED READER. PHERAstar FS Operating Manual BMG LABTECH Figure 6: Unlocking the plate carrier 3. The VICTOR Nivo is a HTRF certified microplate reader. These plates can be read on all HTRF certified readers, thus making their implementation in. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Reagents were dispensed following Cisbio’s ligand binding protocol (Figure 3). FOR HTRF CERTIFIED READER culture plate to a 384-well small volume white plate. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. 7ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Revvity. This protocol, HTS designed, allows miniaturization while. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. 15 / Each of 1; Qty. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. NanoQuant Plate™ allows rapid, straightforward measurement of 16 x 2 µl samples simultaneously without calibration. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). Seal the plate and incubate 3 hours at RT . FOR HTRF CERTIFIED READER. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The ratio between control lysate signal / non-specific signal should be greater than 2. The CulturPlate is PerkinElmer’s microplate designed for cellular assays, tissue-culture treated and sterilized by gamma-irradiation to facilitate adherent cell growth. HTRF is compatible with a large range of available microplate readers. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. The measurement of HTRF assays is predominantly performed on microplate readers. Read & analyse. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Discover our high performance reader. Make sure the setup for Eu Cryptate is used. Assay setup for a 384-well low-volume plate. FOR HTRF CERTIFIED. 4. Make sure the setup for Eu Cryptate is used. NON-SPECIFIC BINDING TOTAL BINDING+ 4 µL HTRF pre-mixed antibodies. With this certification, Cisbio Bioassays guarantees that the Synergy H4 meets or exceeds strict performance and functional criteria for optimal HTRF® readout. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. Not for use in diagnostic procedures. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla (Figure 1). 3. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Make sure the setup for Tb Cryptate is used. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. For the standard curve described, all wells of the microplate are read. 27 / Pack of 100; Qty. Filters or monochromators for emission wavelength. For more information. For more information about HTRF. + 4 µL HTRF pre-mixed antibodies . Specialized filters allow for IR fluorescence. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. FOR HTRF CERTIFIED READER. Spark - Technical Notes. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. Plate 1 plate HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key features BMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. FOR HTRF CERTIFIED READER. This protocol, HTS designed, allows. Microplate reader assays requiring infrared (IR) or. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Add. -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. 96-well culture plateHTRF certified . Plate 25 μL of cells in. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. Part Number# Product Description Number of Plates# 66PL96001 HTRF 96 well low volume plate 1 plate 66PL96005 HTRF 96 well low volume plate 5 platesThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Typical applications The HTRF cartridge provides optimal performance for key applications. If not, our scientific team will help you choose the instrument that best suits your needs and budget. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:320/40 HTRF Eu cryptate 665/7uv Wide Filter 0. But sometimes these parameters are not sufficient enough. Plus . Make sure the setup for Tb Cryptate is used. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. The donor and acceptor can be used to label a variety of biomolecules, for. (donor) of compounds are measured on an HTRF-compatible reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Each well requires 10 μL of 4X lysis buffer /well. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. FOR HTRF CERTIFIED READER For. Our microplates and accessories are manufactured under strict process controls guaranteeing consistent product performance for. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. If not, our scientific team will help you choose the instrument that best suits your needs and budget. For more information about HTRF ®Excitation range. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. 2 Locking the Plate Carrier Upon closing the software or pushing the plate carrier in/out button for more than three seconds (figure 10), the plate carrier will move into the respective lock position. Dedicated UV-laser excitation. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). This protocol gives the cells viability and confluence to be monitored. To perform the assay, use white plate only. Designed to the highest standards to support your drug discovery research, Corning ® and Falcon ® microplates are ideal for drug discovery applications, including assay prep, assay development, and HTS and automation. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Fluorescence intensity – Fluorescence intensity is one of the most. HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed IL1β Antibodies ADD READ ANALYSE Incubate overnight* at room temperature *Reading possibe after 2h White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. Manufacturer. FOR HTRF CERTIFIED READER. Experimental details• HTRF®-Certified Reader **. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for excellence. Revvity. 5% and an increased Z’ value of 0. FOR HTRF CERTIFIED READER. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. HTRF-specific. HTRF-specific. Remove the plate seal and read on an HTRF compatible reader. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator. This protocol gives the cells viability and confluence to be monitored. Synergy H1 offers researchers a choice of monochromators or filters or both detection technologies in one compact unit. • HTRF®-Certified Reader. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. The adapterResults summary for HTRF IP1 standard curve. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Plate 25 μL of cells in. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. HTRF reagent concentrations have been set for optimal assay performances. FOR HTRF CERTIFIED READER For. FOR HTRF CERTIFIED READER For. It measures at any wavelength, with top and bottom reading for most standard technologies. It also integrates with the powerful SoftMax Pro Software for. FOR HTRF CERTIFIED READER. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate AntibodyCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader. Incubate overnight at RT. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. SpectraMax i3/i3x and Paradigm readers can be equipped with. Read & analyse. use of a wide variety of plates or sample matrix formats. The controls are equivalent to the upper and lower limit of. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates. The detection antibody is incubated for 1. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 96-well culture plateFOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. It comes equipped with our patented LVF Monochromators™, filters, and spectrometer. For more ® information about HTRF compatible readers and for set-up recommendations. At Cisbio we provide microplates that help you discover better. The plate was covered both incubated for one hour at room temperature. Microplate Readers and Reader Accessories. Fluorescence intensity – Fluorescence intensity is one of the most popular detection modes, and there are innumerable fluorophores available for applications such as DNA or protein quantitation, reporter-geneCertified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for 21 CFR Part 11. Due to the chemical and photophysical. Partnerships for delivering turn-key solutions. FOR HTRF CERTIFIED READER. Plate 25 μL of cells in. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/vis spectrometer, AAS System, and dedicated AlphaScreen ® and. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. These are the different kinetic modes on BMG LABTECH microplate readers. It can also be further streamlined toLysates are then transferred ®to the assay plate for the detection of Total TYK2 by HTRF reagents. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total Cereblon HTRF detection reagents. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 5 μL of Binding buffer 2X. * For HTRF microplate recommendations, please visit . FOR HTRF CERTIFIED READERThis makes the PHERAstar ®FSX the fastest laser-based TRF reader on the market: 8 seconds for a 96-well plate. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Figure 3. For more information about HTRF. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate Antibody• HTRF®-Certified Reader. Plate 25 μL of cells in. Specialized filters allow for IR fluorescence. View Brochure. . VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the. It accepts all types of microtitration plate. This protocol gives the cells viability and confluence to be monitored. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. + 4 µL HTRF ® pre-mixed antibodies . The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Read & analyse. S. Read & analyse. It also integrates with the. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. * For HTRF microplate recommendations, please visit Table 1 Instrument settings used for HTRF with terbium donor and red acceptor. Make sure the setup for Tb Cryptate is used. Read & analyse. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. Read & analyse. FOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of Total Cofilin by HTRF® reagents. CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-3 (Ser473) by HTRF. For instance, when assay volumes are. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Sensitive, accurate and reliable detection is critical across cell, protein and genomic analysis applications in drug discovery and development. The Z´ value takes into account four (4) parameters: the means (µ) and the standard deviations (σ) of the positive (plateau) and the negative (baseline) controls 1. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. For more information about set-up and compatible HTRF ® readers,• HTRF®-Certified Reader. LANCE ® Ultra DELFIA ® Certified HTRF Readers and Setups Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large. -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. • HTRF®-Certified Reader **. Incubate overnight at RT. Incubate 4h at RT. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 25 μL of cells in. Multimode plate readers include, at the very least, some combination of absorbance (typically covering the UV/visible range), fluorescence and luminescence. Make sure the setup for Eu3+ Cryptate is used. SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. HTRF is compatible with a large range of available microplate readers. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. pre+ 5 µL HTRFcompounds Activation Buffer. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 68. Read & analyse. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. Cells in each image are identified by SoftMax Pro Software and cell-by-cell HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified.